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Enkephalins affect hippocampal membrane phosphorylation.

P R Bär·P Schotman·W H Gispen
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Abstract

Slices of rat brain hippocampus were incubated with methionine-enkephalin, leucine-enkephalin, [Des-Tyr1] methionine-enkephalin or etorphin. After incubation the endogenous phosphorylation of proteins was measured using crude mitochondrial fractions prepared from the incubated slices. Methionine- and leucine-enkephalin specifically enhanced the radiophosphate incorporation into one protein band with an apparent molecular weight of 50K. The effect could also be detected in a fraction containing purified synaptosomal plasma membranes. The effect was time- and dose-dependent and could be mimicked by etorphin and blocked by naloxone. If methionine-enkephalin was added directly to a membrane fraction a marked inhibition of incorporation of phosphate into the 50K band was observed. It is suggested that opiate-receptor activation underlies the effects of enkephalin on hippocampal phosphoprotein metabolism.

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AnimalsCell MembraneEndorphinsEnkephalinsEtorphineHippocampusIn Vitro TechniquesMaleMembranesMitochondriaMolecular WeightNaloxonePhosphorylationRatsSynaptosomesJournal Article

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P R Bär, P Schotman, W H Gispen. 1980-07-25. Enkephalins affect hippocampal membrane phosphorylation.. https://doi.org/10.1016/0014-2999(80)90389-1

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