Plasma membrane isolation on DEAE-Sephadex beads.

L J Gotlib; D B Searls

doi:10.1016/0005-2736(80)90303-x

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PubMed · 7417448

Plasma membrane isolation on DEAE-Sephadex beads.

L J Gotlib·D B Searls

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Abstract

A much-simplified method for the purification of plasma membranes of cultured cells is presented, based upon the attachment of viable cells to nitrocellulose-treated DEAE-Sephadex beads, and their subsequent shearing by hypotonic lysis, agitation on a vortex mixer and sonication. The method is suggested by an older procedure involving attachment to poly-(L-lysine)-coated glass or polyacrylamide beads; the preparation involved in the present method, however, is considerably easier, more rapid and less expensive. Recovery of L-cell plasma membrane marker enzyme activities is approx. 25%, while contamination by internal membrane markers is much less than 1%.

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Animals Cattle Cell Fractionation Cell Membrane Cells, Cultured Chick Embryo Chromatography, Ion Exchange Connective Tissue Mice Journal Article Research Support, U.S. Gov't, P.H.S.

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L J Gotlib, D B Searls. 1980-10-16. Plasma membrane isolation on DEAE-Sephadex beads.. https://doi.org/10.1016/0005-2736(80)90303-x

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