PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 742988

[Methods for working with semi-thin epoxide sections in histological practice].

Iu P Kostilenko·E V Kovalev
View original source ↗Export metadata ↓

Abstract

Since ordinary histological laboratories have no special equipment, semi-thin epoxide embedded sections, to perfectly suitable to realize the resolving capacity of the light optic are not widely used in histological practice. However, even in an ordinary histological laboratory it is possible to master the method of their production. A special device is suggested to fix glass knives in the rotational microtome MPC-2. Simultaneously, a number of suggestions and recommendations are given as to certain specificities for obtaining serial semi-thin sections and the method for their staining. Besides, an original method is offered for producing film preparations by means of mild tissue compression in the process of polymerization in epoxide resin. This method allows not only to study some features in the composition of complex three-dimentional objects, but to obtain a series of semi-thin sections from the film preparation to reveal intimal interconnections between certain structural components.

Explore related subjects

Epoxy ResinsMicrotomyEnglish AbstractJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Iu P Kostilenko, E V Kovalev. 1978. [Methods for working with semi-thin epoxide sections in histological practice].. https://pubmed.ncbi.nlm.nih.gov/742988/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2006

The effect of changes in surface wettability on two-phase saturated flow in horizontal replicas of single natural fractures.

By using translucent epoxy replicas of natural single fractures, it is possible to optically measure aperture distribution and directly observe NAPL flow. However, detailed characterization of epoxy reveals that it is not a sufficiently good analogue to natural rock for many two-phase flow studies. The surface properties of epoxy, which is hydrophobic, are quite unlike those of natural rock, which is generally assumed to be hydrophilic. Different surface wettabilities result in dramatically different two-phase flow behavior and residual distributions. In hydrophobic replicas, the NAPL flows in well-developed channels, displacing water and filling all of the pore space. In hydrophilic replicas, the invading NAPL is confined to the largest aperture pathways and flow frequently occurs in pulses, with no limited or no stable channel development, resulting in isolated blobs with limited accessible surface area. The pulsing and channel abandonment behaviors described are significantly different from the piston-flow frequently assumed in current modeling practice. In addition, NAPL never achieved total saturation in hydrophilic models, indicating that significantly more than a monolayer of water was bound to the model surface. Despite typically only 60-80% NAPL saturation, there was generally good agreement between theoretically calculated Young-Laplace aperture invasion boundaries and the observed minimum apertures invaded. The key to determining whether surface wettability is negligible, or not, lies in accurate characterization of the contaminant-geologic media system under study. As long as the triple-point contact angle of the system is low (<20 degrees), the assumption of perfect water wettability is not a bad one.

Epoxy Resins↗
PubMed2005

Fabrication of enclosed SU-8 tips for electrospray ionization-mass spectrometry.

We describe a novel electrospray tip design for MS which is fabricated completely out of SU-8 photoepoxy. A three-layer SU-8 fabrication process provides fully enclosed channels and tips. The tip shape and alignment of all SU-8 layers is done lithographically and is therefore very accurate. Fabrication process enables easy integration of additional fluidic functions on the same chip. Separation channels can be made with exactly the same process. Fluidic inlets are made in SU-8 during the fabrication process and no drilling or other postprocessing is needed. Channels have been fabricated and tested in the size range of 10 microm x 10 microm-50 microm x 200 microm. Mass spectrometric performance of the tips has been demonstrated with both pressure-driven flow and EOF. SU-8 microtips have been shown to produce stable electrospray with EOF in a timescale of tens of minutes. With pressure driven flow stable spray is maintained for hours. Taylor cone was shown to be small in volume and well defined even with the largest channel cross section. The spray was also shown to be well directed with our tip design.

Epoxy Resins↗