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PubMed · 7430506

Polymerized insoluble bee venom.

R Patterson·I M Suszko·S Hendrix·C R Zeiss·J J Pruzansky
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Abstract

Using a polymerization process previously used for ragweed allergens, honeybee venom was polymerized. Instead of soluble polymers, an insoluble precipitate, polymerized insoluble bee venom (PIBV), is the result. A major allergen of honeybee venom, 125I phospholipase A (PL-A) incorporated into PIBV, was shown to have decreased dissemination from subcutaneous injection sites. After thorough mixing, samples of PIBV can be withdrawn from a vial with a syringe with no more than 10% error. Approximately 90% of PL-A was incorporated with PIBV. The soluble PL-A was removed during subsequent washing of the PIBV and this soluble PL-A was shown to be polymerized mainly to high-molecular weight PL-AS. PIBV (20 mg) injected in each of six rabbits resulted in formation of precipitating antibody in all rabbits. The rabbit antisera bound 125I PL-A and reacted with PL-A in whole been venom. These physical and immunologic characteristics of PIBV suggest further study of its potential for human use.

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AbsorptionAnimalsAntibody FormationBee VenomsBinding SitesGuinea PigsInjections, SubcutaneousIodine RadioisotopesPhospholipases APolymersRabbitsSolubilityComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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R Patterson, I M Suszko, S Hendrix, C R Zeiss, J J Pruzansky. 1980. Polymerized insoluble bee venom.. https://doi.org/10.1016/0091-6749(80)90011-1

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