PubMed HealthSearch

PubMed · 7436549

Evaluating promulgated refinery effluent standards using artificial streams.

Abstract

Two-week tests on an Artificial Refinery MIxture (ARM) were conducted on periphyton communities on glass slides in laboratory streams. ARM components, which are present in "typical" refinery effluents, approximate maximum allowable concentrations as promulgated by the 1977 U.S. Environmental Protection Agency (USEPA) guidelines for refinery effluents. ARM concentrations tested ranged from approximately 0.25 to 5 times full strength. The nontaxonomic structural parameters--ash-free dry weight, chlorophyll a, and ATP--indicated that ARM concentrations of 1 to 5X stimulated periphyton colonization rates. ARM concentrations of 0.25 to 5X caused a shift in dominant periphytic algae from diatoms in reference streams to green algae in dosed streams; this resulted in higher pH levels in reference streams, apparently related to photosynthetic activity. Changes in nontaxonomic structural parameters of slides precolonized with periphyton were not sensitive water quality indicators. Dry weight was not a useful biomass indicator due to high levels of suspended solids in ARM. The promulgated 1977 guidelines may be adequate to maintain nontaxonomic structural integrity of periphyton communities, while the 1983 guidelines appear to be adequate to maintain taxonomic integrity.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

R A Honig, A L Buikema. 1980. Evaluating promulgated refinery effluent standards using artificial streams.. https://doi.org/10.1007/bf01056940

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

The human mitochondrial genome contains a second light strand promoter.

The human mitochondrial genome must be replicated and expressed in a timely manner to maintain energy metabolism and supply cells with adequate levels of adenosine triphosphate. Central to this process is the idea that replication primers and gene products both arise via transcription from a single light strand promoter (LSP) such that primer formation can influence gene expression, with no consensus as to how this is regulated. Here, we report the discovery of a second light strand promoter (LSP2) in humans, with features characteristic of a bona fide mitochondrial promoter. We propose that the position of LSP2 on the mitochondrial genome allows replication and gene expression to be orchestrated from two distinct sites, which expands our long-held understanding of mitochondrial gene expression in humans.

Adenosine Triphosphate

GCN2 kinase activation by ATP-competitive kinase inhibitors.

Small-molecule kinase inhibitors represent a major group of cancer therapeutics, but tumor responses are often incomplete. To identify pathways that modulate kinase inhibitor response, we conducted a genome-wide knockout (KO) screen in glioblastoma cells treated with the pan-ErbB inhibitor neratinib. Loss of general control nonderepressible 2 (GCN2) kinase rendered cells resistant to neratinib, whereas depletion of the GADD34 phosphatase increased neratinib sensitivity. Loss of GCN2 conferred neratinib resistance by preventing binding and activation of GCN2 by neratinib. Several other Food and Drug Administration (FDA)-approved inhibitors, such erlotinib and sunitinib, also bound and activated GCN2. Our results highlight the utility of genome-wide functional screens to uncover novel mechanisms of drug action and document the role of the integrated stress response (ISR) in modulating the response to inhibitors of oncogenic kinases.

Adenosine Triphosphate