PubMed · 7450828
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S P Amin, B V Mantri, K N Sahgal. 1980. Glucose analysis methods; a one more comparison.. https://pubmed.ncbi.nlm.nih.gov/7450828/
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A simple and rapid analytical assay for determination of total cholesterol, HDL-cholesterol and triglycerides in capillary blood samples has been compared with established laboratory methods in a group of 41 test subjects. The new method produces results that are fully acceptable for determination of these three lipid parameters, and agree well with the results obtained in serum by established laboratory methods. The Cholestech LDX analyser may be useful for providing rapid and accurate measurements of the lipid profile, including total cholesterol, triglycerides and HDL-cholesterol. Its small size, the speed of analysis and the fact that it is easy to use make the Cholestech LDX a suitable instrument for use in out-patient clinics and primary care medicine.
Serum 1,5-anhydro-D-glucitol (AG) has been proposed as a marker of glycaemic control in diabetic patients. Two methods have been developed which could be applied to routine clinical monitoring of serum AG. We have compared the assay characteristics of an adapted enzyme assay, based on the enzyme pyranose oxidase, with a high-performance anion-exchange chromatographic method using pulsed amperometric detection (HPAEC-PAD). Linearity and minimum detectable concentrations were practically identical (to at least 400 mumol/l and 4 mumol/l AG, respectively), though intra- and inter-assay precision was better with the HPAEC PAD system at a clinically relevant concentration of 40 mumol/l AG (9.8% vs. 11.8% and 9.2% vs. 13.0%, respectively). The recovery of added AG to serum samples was lower with the micro-enzyme assay than the HPAEC-PAD assay (74 +/- 15% vs. 102 +/- 8%). Despite good agreement by linear regression (r = 0.974, P < 0.005), the assay methods did not demonstrate satisfactory agreement when using a difference plot (limits of agreement -16.1 to 18.7 mumol/l). We conclude that although both assay methods are applicable to routine analyses, the HPAEC-PAD is more precise and is more specific for AG than the enzyme based assay.
OBJECTIVE: To evaluate the analytical performance of the i-STAT system, which is designed for point of care testing and employs a hand-held chemistry analyzer and disposable cartridges, which in the configuration tested, are capable of measuring sodium, potassium, chloride, urea, glucose, and hematocrit in approximately 65 microL of blood in 90's. METHODS: Linearity and imprecision in hematocrit measurement were assessed using whole blood, while that for the other analytes were evaluated with aqueous solutions. The accuracy of the i-STAT system was judged by assay of patient specimens obtained both by venipuncture and fingerprick and correlated with the Kodak Ektachem 700XR and the microhematocrit methods. RESULTS: Linearity was obtained over the clinically relevant range for all analytes. Total imprecision as expressed by the coefficient of variation (CV) was less than 3.5% for all analytes at both high and low concentration except for a low concentration of urea where a CV of 9.4% was obtained. Linear regression analysis revealed minimal systematic errors. The standard error about the regression line (Sy/x) ranged from 0.017 for hematocrit to 2.262 for chloride in the assay of venous blood, whereas in the assay of capillary blood the Sy/x ranged from 0.018 for hematocrit to 0.755 for glucose. CONCLUSIONS: The analytical performance of the i-STAT was deemed acceptable by calculation of total error and comparison with published performance standards. Our study has shown the i-STAT system to be reliable, robust, and simple to operate. Moreover, the compactness of the analyzer and the requirement for only small volumes of whole blood will make it a valuable diagnostic tool in point of care settings.