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PubMed · 7459361

Does random collisional cross-linking occur?

T H Ji·C R Middaugh
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Abstract

In fluid membranes, mobile molecules are thought to collide at high frequencies. Concern has been expressed as to whether these colliding molecules are cross-linking during the chemical cross-linking of membrane molecules, thereby creating problems in interpreting such experiments. Hemoglobin was used as a model to test this possibility. Oligomers larger than the tetramer could be cross-linked depending on factors such as hemoglobin concentration, duration of the cross-linking reaction and the type of reagent. Under certain conditions, however, such as a hemoglobin concentration less than 150 microM or a duration of cross-linking shorter than 15 min, larger oligomers were not detectable. Analysis of these data suggests that the probability of random collisional cross-links under normal conditions is insignificant.

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Chemical PhenomenaChemistryCross-Linking ReagentsHemoglobinsHumansMembrane FluidityProtein ConformationJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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T H Ji, C R Middaugh. 1980-12-12. Does random collisional cross-linking occur?. https://doi.org/10.1016/0005-2736(80)90382-x

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