A new procedure for analysing the relationship between different cell surface antigens.

C R Parish; H C O'Neill; I F McKenzie

doi:10.1016/0022-1759(80)90057-5

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A new procedure for analysing the relationship between different cell surface antigens.

C R Parish·H C O'Neill·I F McKenzie

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Abstract

An immunoprecipitation-inhibition procedure is assessing whether different antigenic determinants are carried on the same molecule or on different molecules on cells. The procedure entails (a) exposing NP-40 lysates of cells to antibody against one antigenic specificity; (b) removing free antibody and immune complexes by absorption with protein A-bearing S. aureus bacteria; (c) adsorption of the NP-40 with a detergent binding resin; and (d) measuring the inhibitory activity of the lysates for antibody against another specificity by a rosetting assay. This method has several advantages over the widely used sequential immunoprecipitation procedure.

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Animals Antigens, Surface Cell Membrane Chemical Precipitation Detergents Female Isoantigens Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Spleen Journal Article Research Support, Non-U.S. Gov't

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C R Parish, H C O'Neill, I F McKenzie. 1980. A new procedure for analysing the relationship between different cell surface antigens.. https://doi.org/10.1016/0022-1759(80)90057-5

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