RNA structural patterns and splicing: molecular basis for an RNA-based enhancer.

D Libri; F Stutz; T McCarthy; M Rosbash

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PubMed · 7493320

RNA structural patterns and splicing: molecular basis for an RNA-based enhancer.

D Libri·F Stutz·T McCarthy·M Rosbash

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Abstract

Efficient splicing of the 325-nt yeast (Saccharomyces cerevisiae) rp51b intron requires the presence of two short interacting sequences located 200 nt apart. We used the powerful technique of randomization-selection to probe the overall structure of the intron and to investigate its role in pre-mRNA splicing. We identified a number of alternative RNA-RNA interactions in the intron that promote efficient splicing, and we showed that similar base pairings can also improve splicing efficiency in artificially designed introns. Only a very limited amount of structural information is necessary to create or maintain such a mechanism. Our results suggest that the base pairing contributes transiently to the spliceosome assembly process, most likely by complementing interactions between splicing factors. We propose that splicing enhancement by structure represents a general mechanism operating in large yeast introns that evolutionarily preceded the protein-based splicing enhancers of higher eukaryotes.

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Base Sequence Cloning, Molecular Introns Molecular Sequence Data Mutagenesis, Site-Directed Nucleic Acid Conformation RNA RNA Precursors RNA Splicing RNA, Fungal RNA, Messenger Ribosomal Proteins Saccharomyces cerevisiae Selection, Genetic Sequence Analysis, DNA Transformation, Genetic Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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D Libri, F Stutz, T McCarthy, M Rosbash. 1995. RNA structural patterns and splicing: molecular basis for an RNA-based enhancer.. https://pubmed.ncbi.nlm.nih.gov/7493320/

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