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PubMed · 7496531

The bacteriophage 434 operator/repressor system in yeast.

C I Webster·W J Brammar
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Abstract

The ability of the bacteriophage 434 operator/repressor system to function in a eukaryotic cell has been explored. An idealized 434 operator was placed at various positions in the PGK promoter of Saccharomyces cerevisiae: within the upstream activator sequence, close to the TATA box, and downstream of the transcription-initiation site. Expression of the 434 cI gene from a 2 microns-based plasmid resulted in significant repression of gene expression from constructs containing the altered promoters linked to a beta-galactosidase reporter gene. Attempts to use a variant of the 434 repressor that has the binding specificity of the P22 repressor (434P22) were unsuccessful, due to a severely inhibitory effect of this gene-product on the growth of the yeast cells.

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Bacteriophage P22Base SequenceCloning, MolecularColiphagesDNA-Binding ProteinsFungal ProteinsGenes, FungalGenes, ViralGenetic VectorsMolecular Sequence DataMutagenesis, Site-DirectedOperator Regions, GeneticPhosphoglycerate KinasePromoter Regions, GeneticRecombinant Fusion ProteinsRepressor ProteinsSaccharomyces cerevisiaeViral ProteinsViral Regulatory and Accessory Proteinsbeta-GalactosidaseJournal ArticleResearch Support, Non-U.S. Gov't

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C I Webster, W J Brammar. 1995. The bacteriophage 434 operator/repressor system in yeast.. https://doi.org/10.1099/13500872-141-9-2191

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