GDNF: existence of a second transcript in the brain.

N Cristina; C Chatellard-Causse; M Manier; C Feuerstein

doi:10.1016/0169-328x(95)00103-y

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GDNF: existence of a second transcript in the brain.

N Cristina·C Chatellard-Causse·M Manier·C Feuerstein

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Abstract

The detection of the glial cell-line derived neurotrophic factor (GDNF) mRNA by RT-PCR in dissociated cell culture of rat embryonic or post-natal brain allowed the amplification of a doublet. The major band corresponded to the expected size and the minor one to a shorter product. We cloned and sequenced the latter product, and thus identified a mRNA potentially encoding for an isoform of the initially described precursor protein involved in GDNF synthesis.

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Animals Base Sequence Brain Cells, Cultured Cloning, Molecular Glial Cell Line-Derived Neurotrophic Factor Molecular Sequence Data Nerve Growth Factors Nerve Tissue Proteins Polymerase Chain Reaction RNA, Messenger Rats Rats, Inbred Strains Sequence Analysis Transcription, Genetic Journal Article

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N Cristina, C Chatellard-Causse, M Manier, C Feuerstein. 1995. GDNF: existence of a second transcript in the brain.. https://doi.org/10.1016/0169-328x(95)00103-y

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