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Separation of origin recognition complex functions by cross-species complementation.

A E Ehrenhofer-Murray·M Gossen·D T Pak·M R Botchan·J Rine
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Abstract

Transcriptional silencing at the HMRa locus of Saccharomyces cerevisiae requires the function of the origin recognition complex (ORC), the replication initiator of yeast. Expression of a Drosophila melanogaster Orc2 complementary DNA in the yeast orc2-1 strain, which is defective for replication and silencing, complemented the silencing defect but not the replication defect; this result indicated that the replication and silencing functions of ORC were separable. The orc2-1 mutation mapped to the region of greatest homology between the Drosophila and yeast proteins. The silent state mediated by DmOrc2 was epigenetic; it was propagated during mitotic divisions in a relatively stable way, whereas the nonsilent state was metastable. In contrast, the silent state was erased during meiosis.

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AnimalsCloning, MolecularDNA ReplicationDNA-Binding ProteinsDrosophila ProteinsDrosophila melanogasterFungal ProteinsGene Expression RegulationGenes, FungalGenes, InsectGenetic Complementation TestMutationOrigin Recognition ComplexRegulatory Sequences, Nucleic AcidReplication OriginRepressor ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTemperatureTransformation, GeneticJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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A E Ehrenhofer-Murray, M Gossen, D T Pak, M R Botchan, J Rine. 1995-12-08. Separation of origin recognition complex functions by cross-species complementation.. https://doi.org/10.1126/science.270.5242.1671

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