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Target cells modulate dopamine transporter gene expression during brain development.

C Perrone-Capano·A Tino·U di Porzio
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Abstract

We have analysed the expression of the dopamine transporter (DAT) gene and compared it with that of tyrosine hydroxylase, neuronal GABA transporter and synaptic vesicle monoamine transporter genes during pre- and post-natal development of rat mesencephalic dopaminergic (DA) neurones. Our results show that DAT transcripts are not detectable until embryonic day (E) 15, whilst those of the other genes analysed are already present at E12. In vitro, the level of DAT gene transcription in mesencephalic E13 DA neurones is increased in coculture with target striatal cells. Thus striatal targets cells regulate, at the transcriptional level, a key step of dopaminergic neurotransmission during DA neurone development.

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AnimalsBase SequenceBrainCarrier ProteinsCells, CulturedDopamineDopamine Plasma Membrane Transport ProteinsElectrochemistryGene ExpressionMembrane GlycoproteinsMembrane Transport ProteinsMesencephalonMolecular Sequence DataNerve Tissue ProteinsPolymerase Chain ReactionProton-Translocating ATPasesRNA-Directed DNA PolymeraseRatsSynaptic TransmissionTyrosine 3-MonooxygenaseJournal ArticleResearch Support, Non-U.S. Gov't

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C Perrone-Capano, A Tino, U di Porzio. 1994-05-09. Target cells modulate dopamine transporter gene expression during brain development.. https://doi.org/10.1097/00001756-199405000-00031

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