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PubMed · 7522294

Leukemia-associated changes identified by quantitative flow cytometry. II. CD5 over-expression and monitoring in B-CLL.

T Lavabre-Bertrand·G Janossy·C Exbrayat·P Bourquard·C Duperray·M Navarro
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Abstract

The CD5 antigen density on B cells was studied on fetal spleen, cord blood, and adult peripheral blood (after immunomagnetic bead purification) using an indirect immunofluorescence technique. In fetal spleen, there was a continuum in CD5 expression, whereas all cord blood and less than 20% adult peripheral blood B cells were CD5+. Mean CD5 antigen density on these normal cells was low (3-6 x 10(3) molecules/cell); eight to 20 times lower than on normal T lymphocytes. In adult blood, less than 10% B cells expressed more than 3 x 10(3) CD5 molecules/cell. In chronic malignancies, 34/35 cases had a CD5 antigen density lower than on residual T cells, but mean antigen density was higher (14.8 +/- 2.1 x 10(3) molecules/cell) than on normal B cells. Sixteen cases of chronic lymphocytic leukemia (50%) expressed a CD5 density above 10 x 10(3) molecules/cell. This aberrantly high CD5 expression was used to detect neoplastic cells after dilution in normal lymphocytes, with a limit of detection between 1:100 and 1:1000. Quantitation of the CD5 antigen allows better characterization of the B1 population and should be used for the monitoring of chronic malignancies.

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Antigens, CDB-LymphocytesBiomarkers, TumorCD5 AntigensFetal BloodFlow CytometryFluorescent Antibody TechniqueHumansLeukemia, Lymphocytic, Chronic, B-CellSpleenJournal ArticleResearch Support, Non-U.S. Gov't

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T Lavabre-Bertrand, G Janossy, C Exbrayat, P Bourquard, C Duperray, M Navarro. 1994. Leukemia-associated changes identified by quantitative flow cytometry. II. CD5 over-expression and monitoring in B-CLL.. https://pubmed.ncbi.nlm.nih.gov/7522294/

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