Hypervariable epitope constructs as a means of accounting for epitope variability.

D E Anderson; A Malley; E Benjamini; M B Gardner; J V Torres

doi:10.1016/0264-410x(94)90225-9

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Hypervariable epitope constructs as a means of accounting for epitope variability.

D E Anderson·A Malley·E Benjamini·M B Gardner·J V Torres

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Abstract

Epitope variability is one of the greatest obstacles to development of synthetic peptide vaccines. Based on a recently described hypervariable epitope (aa 414-434) on the envelope glycoprotein (gp130) to simian immunodeficiency virus (SIVmac142), we have developed a novel approach to account for epitope variability. We have prepared, in a single synthesis, a cocktail of peptides, designated a hypervariable epitope construct (HEC), which collectively represent all the in vivo variability seen in an epitope. The HEC represents permutations of amino acid substitutions found in the epitope and has been able to induce antibodies with enhanced binding to native SIV and broad immunoreactivity to related epitope analogues.

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Amino Acid Sequence Animals Enzyme-Linked Immunosorbent Assay Epitopes Gene Products, env Macaca mulatta Mice Mice, Inbred BALB C Molecular Sequence Data Peptides Simian Immunodeficiency Virus Vaccines, Synthetic Viral Vaccines Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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D E Anderson, A Malley, E Benjamini, M B Gardner, J V Torres. 1994. Hypervariable epitope constructs as a means of accounting for epitope variability.. https://doi.org/10.1016/0264-410x(94)90225-9

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