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Functional interaction between the epidermal growth factor receptor and c-Src kinase activity.

P A Oude Weernink·A E Ottenhoff-Kalff·M P Vendrig·E A van Beurden·G E Staal·G Rijksen
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Abstract

To study the relationship between the tyrosine kinase c-Src and the epidermal growth factor receptor (EGF-R), we used the breast cancer cell line ZR75-1, which was transfected with the EGF-R. The EGF-R transfected cell line expressed 60 times more EGF-R than a control cell line transfected with the empty vector. In the presence of EGF, the EGF-R over-expressing cell line grew much faster than the control cell line. Both cell lines expressed approximately equal amounts of c-Src. However, the cell line over-expressing the EGF-R showed a twofold enhancement of c-Src kinase activity after EGF stimulation. The activation of c-Src kinase by EGF was confirmed in other EGF-R expressing cell types.

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Breast NeoplasmsCell LineCell MembraneCytosolEpidermal Growth FactorErbB ReceptorsHumansKineticsProtein-Tyrosine KinasesProto-Oncogene Proteins pp60(c-src)Recombinant ProteinsTransfectionTumor Cells, CulturedJournal Article

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P A Oude Weernink, A E Ottenhoff-Kalff, M P Vendrig, E A van Beurden, G E Staal, G Rijksen. 1994-10-03. Functional interaction between the epidermal growth factor receptor and c-Src kinase activity.. https://doi.org/10.1016/0014-5793(94)00977-5

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