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Differences in the response to mutagens between two V79 sublines.

G Speit·B Habermeier·R Helbig
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Abstract

Two V79 Chinese hamster sublines (V79-UL and V79-MZ) which differed markedly with respect to their spontaneous pattern of mutations at the HPRT locus were comparatively investigated in genotoxicity tests with ethyl methanesulphonate (EMS). EMS-induced frequencies of HPRT mutations and sister chromatid exchanges (SCEs) were much higher in V79-MZ than in V79-UL. V79-MZ were not hypersensitive against EMS and had normal frequencies of spontaneous gene mutations and chromosome aberrations. Baseline SCE frequencies at various BrdUrd concentrations were slightly increased compared to V79-UL. EMS induced a similar amount of chromosome aberrations in both cell lines but exchange figures occurred with lower frequency in V79-MZ. The results indicate that specific and significant differences in the response to mutagens may exist between 'normal' Chinese hamster cell lines which might be relevant for genotoxicity testing.

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AnimalsCell LineCricetinaeCricetulusEthyl MethanesulfonateHypoxanthine PhosphoribosyltransferaseMutagenicity TestsMutagensSister Chromatid ExchangeJournal ArticleResearch Support, Non-U.S. Gov't

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G Speit, B Habermeier, R Helbig. 1994. Differences in the response to mutagens between two V79 sublines.. https://doi.org/10.1016/0165-7992(94)90009-4

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