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PubMed · 7527678

Adhesion molecules in hematopoietic cells.

T Kinashi·T A Springer
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Abstract

Interaction with stromal cells is known to be crucial for growth and differentiation of hematopoietic cells. To characterize adhesion molecules involved in this interaction, we examined adhesion of a panel of lymphoid, myeloid, and mast cell lines with stromal cells. We found that very late antigen-4 (VLA-4) and vascular cell adhesion molecule 1 (VCAM-1) were major adhesion molecules in lymphoid and myeloid cells, whereas myeloma cells adhered to stromal cells through hyaluronate. We investigated regulation of VLA-4 during differentiation of myeloid cells using a neutrophil precursor cell line, L-G3. Differentiation of neutrophils induced by granulocyte colony-stimulating factor was accompanied with down-regulation of VLA-4. Induced L-G3 cells adhered to stromal cells in proportion to the expression of VLA-4. Mast cells used two mechanisms to adhere to fibroblasts and stromal cells. They adhered to fibronectin through VLA-5 when stimulated with steel factor and also directly to membrane-anchored steel factor through c-kit.

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AnimalsCell AdhesionCell Adhesion MoleculesCell DifferentiationCell LineDown-RegulationHematopoietic Cell Growth FactorsHematopoietic Stem CellsMiceMice, Inbred BALB CNeutrophilsProto-Oncogene ProteinsProto-Oncogene Proteins c-kitReceptor Protein-Tyrosine KinasesReceptors, Colony-Stimulating FactorReceptors, Very Late AntigenStem Cell FactorStromal CellsTumor Cells, CulturedVascular Cell Adhesion Molecule-1Journal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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T Kinashi, T A Springer. 1994. Adhesion molecules in hematopoietic cells.. https://pubmed.ncbi.nlm.nih.gov/7527678/

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