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PubMed · 7528102

Perlecan, basal lamina proteoglycan, promotes basic fibroblast growth factor-receptor binding, mitogenesis, and angiogenesis.

D Aviezer·D Hecht·M Safran·M Eisinger·G David·A Yayon
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Abstract

A survey of defined species of cell surface and extracellular matrix heparan sulfate proteoglycans (HSPG) was performed in a search for cellular proteoglycans that can promote bFGF receptor binding and biological activity. Of the various affinity-purified HSPGs tested, perlecan, the large basement membrane HSPG, is found to induce high affinity binding of bFGF both to cells deficient in HS and to soluble FGF receptors. Heparin-dependent mitogenic activity of bFGF is strongly augmented by perlecan. Monoclonal antibodies to perlecan extract the receptor binding promoting activity from active HSPG preparations. In a rabbit ear model for in vivo angiogenesis, perlecan is a potent inducer of bFGF-mediated neovascularization. These results identify perlecan as a major candidate for a bFGF low affinity, accessory receptor and an angiogenic modulator.

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AnimalsBasement MembraneCHO CellsCell DivisionCricetinaeDose-Response Relationship, DrugEarExtracellular MatrixFemaleFibroblast Growth Factor 2Heparan Sulfate ProteoglycansHeparan SulfateHumansLungNeovascularization, PathologicProtein BindingProteoglycansRabbitsReceptors, Fibroblast Growth FactorJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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D Aviezer, D Hecht, M Safran, M Eisinger, G David, A Yayon. 1994-12-16. Perlecan, basal lamina proteoglycan, promotes basic fibroblast growth factor-receptor binding, mitogenesis, and angiogenesis.. https://doi.org/10.1016/0092-8674(94)90031-0

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