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PubMed · 7528691

Carbohydrate moieties on sperm surface: physiological relevance.

M Kurpisz·N J Alexander
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Abstract

OBJECTIVES: To study the cross-reactions between mouse monoclonal antisperm antibodies and somatic cells or bacteria, to identify the antigenic determinants responsible for such cross-reactions, and to correlate between the antibody function and determinant recognition. DESIGN: Activities of monoclonal antibodies (mAbs) were characterized by immunosorbent assay of RIA technique; sperm epitopic characterization was performed in lectin-blocking and sugar competitive assays and correlated with functional assays. SETTING: Procedures were performed in a university laboratory. RESULTS: The extensive cross-reactivity between antigenic determinants of sperm, erythrocytes, and bacteria (but not bacterial deglycosylated lipopolysaccharides) was observed. The analytic procedures indicated predominant mAb reactions to carbohydrates such as fucose, galactose, mannose, N-acetylglucosamine and N-acetylgalactosamine. Approximately half of the 30 tested mAbs interfered in the functional assays, that is, sperm agglutination, immobilization, and zona-free penetration. CONCLUSIONS: Sperm carbohydrates seem to induce antibody reactions to common antigenic determinant(s) present on gametes, somatic cells, and infectious agents. Thus molecular mimicry between bacteria and sperm can be a major factor inducing antisperm immunological reactions. Obtained antisperm mAbs, reacting to glycosylated epitopes, presented very strong properties in sperm agglutination and/or immobilization. This did not correlate with inhibiting properties of some antibodies in xenogeneic zona-free penetration test, that is, this assay possibly is not based on oligosaccharide mediation.

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AnimalsAntibodies, MonoclonalBacteriaCarbohydrate MetabolismCarbohydratesCell MembraneCross ReactionsEpitopesErythrocytesFemaleHumansLectinsMaleMiceSperm AgglutinationSperm MotilitySperm-Ovum InteractionsSpermatozoaJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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M Kurpisz, N J Alexander. 1995. Carbohydrate moieties on sperm surface: physiological relevance.. https://pubmed.ncbi.nlm.nih.gov/7528691/

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