Modified RNA sequence pools for in vitro selection.

Y Lin; Q Qiu; S C Gill; S D Jayasena

doi:10.1093/nar/22.24.5229

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Modified RNA sequence pools for in vitro selection.

Y Lin·Q Qiu·S C Gill·S D Jayasena

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Abstract

We report the use of modified RNA, in which the 2'-OH group of pyrimidines is replaced by a 2'-amino (2'-NH2) group to identify high affinity ligands specific for human neutrophil elastase (HNE) by in vitro selection. Compared to unmodified RNA the 2'-NH2-modified RNA ligands show enhanced stability in human serum and urine. Use of RNase T1 cleavage data in the presence of K+ and Li+ ions suggests that the modified RNA ligands selected for HNE form an intermolecular G-quartet structure.

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Amino Acid Sequence Base Sequence Humans Hydrogen-Ion Concentration Kinetics Leukocyte Elastase Ligands Molecular Sequence Data Nucleic Acid Conformation Pancreatic Elastase RNA Radioligand Assay Journal Article

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Y Lin, Q Qiu, S C Gill, S D Jayasena. 1994-12-11. Modified RNA sequence pools for in vitro selection.. https://doi.org/10.1093/nar%2F22.24.5229

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