PubMed Health⌕ Search

PubMed · 7529513

Generating customized, long-lived 32P-labeled RNA size markers.

Abstract

The source did not provide an abstract. Follow the original record for more information.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

P Liggit, S H Cheng, E J Baker. 1994. Generating customized, long-lived 32P-labeled RNA size markers.. https://pubmed.ncbi.nlm.nih.gov/7529513/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

Radioisotope studies of root activity and root-level interactions in tree-based production systems: a review.

This review focuses on the radioisotope methods used for studying the root activity patterns of woody perennials and root competition in multi-species cropping systems. 32P soil-injection is by far the most widely used method. The method is unique as it enables one to delineate the lateral and vertical spread of the absorbing roots. A system of classification of crop plants based on the concept of 'effective foraging space' has been developed. The scope and applicability of the radioisotope methods in different cropping situations as well as from the point of view of radiological safety were discussed.

Phosphorus Radioisotopes↗

An RNA ligase from wheat germ which participates in transfer RNA splicing in vitro.

Transfer RNA half-molecules are intermediates in the splicing of tRNA precursors containing intervening sequences. We have utilized yeast tRNA half-molecules to identify and partially purify an ATP-dependent RNA ligase activity from extracts of wheat germ. This activity can complement a yeast tRNA endonuclease in vitro to efficiently splice 10 different yeast tRNA precursors. The products of in vitro splicing are a covalently joined tRNA and a circular intervening sequence RNA. The internucleotide bond formed at the splice junction is a 2'-phosphomonoester, 3',5'-phosphodiester structure. The 2'-phosphate originates from the 2',3'-cyclic phosphate at the 3' terminus of the 5' half-tRNA. The phosphodiester phosphate is derived from the gamma-phosphate of ATP.

Phosphorus Radioisotopes↗