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PubMed · 7529532

Interleukin-3 administration enhances human monocyte function in vivo.

A Khwaja·I E Addison·B Johnson·K Yong·D C Linch
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Abstract

In addition to its haemopoietic effects, interleukin-3 (IL-3) enhances leucocyte function in vitro. In this study we examined the effects on haematological variables and monocyte function of a single IL-3 infusion in five haematologically normal individuals. There was a rapid fall in circulating monocyte (to 24 +/- 6% of pre-infusion value) and eosinophil numbers (to 3 +/- 2%) with a nadir at 30 min and gradual return to baseline over 6 h. No significant changes in monocyte expression of the adhesion molecules CD11b or L-selectin or of monocyte respiratory burst activity were detected. There was a significant increase in monocyte phagocytosis and killing of Candida after IL-3 infusion: the percentage of monocytes which had ingested Candida increased from 39 +/- 10% to 62 +/- 12% and the total number of Candida killed per 100 monocytes increased from 63 +/- 34 to 210 +/- 59 (P < 0.05 and P < 0.01 respectively). There was no inhibition of neutrophil migration into a 'skin window' site and monocyte migration was moderately enhanced (peak increase of 260 +/- 47%). These results show that IL-3 has significant effects on monocyte function in vivo and could be of use in augmenting host defence mechanisms in immunocompromised patients.

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CD11 AntigensCell Adhesion MoleculesCell MovementEnzyme-Linked Immunosorbent AssayHumansInterleukin-3L-SelectinLeukocyte CountMonocytesNeutrophilsPhagocytosisRespiratory BurstJournal ArticleResearch Support, Non-U.S. Gov't

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A Khwaja, I E Addison, B Johnson, K Yong, D C Linch. 1994. Interleukin-3 administration enhances human monocyte function in vivo.. https://doi.org/10.1111/j.1365-2141.1994.tb05067.x

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