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PubMed · 7544191

Preparation of cDNA-silica using reverse transcriptase and its DNA sequence determination.

H W Jarrett
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Abstract

A new method for producing macroporous silica (suitable for high-performance liquid chromatography) with covalently attached DNA is presented. The method uses (dT)18-silica as a primer, annealed to a poly(A)-RNA template, which is then transcribed using reverse transcriptase. The RNA template is eluted and single-stranded cDNA-silica is recovered. The cDNA-silica can be sequenced using the dideoxy method. These methods provide a facile method for producing cDNA-silica of demonstrable authenticity and provide a unique approach to DNA and RNA sequencing.

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Base SequenceDNA, ComplementaryMolecular Sequence DataRNA-Directed DNA PolymeraseSequence Analysis, DNASilicon DioxideJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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H W Jarrett. 1995-07-28. Preparation of cDNA-silica using reverse transcriptase and its DNA sequence determination.. https://doi.org/10.1016/0021-9673(95)00382-w

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