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PC12-E2 cells: a stable variant with altered responses to growth factor stimulation.

Y Y Wu·R A Bradshaw
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Abstract

A variant cell line, designated E2, characterized by more rapid responses to nerve growth factor (NGF) and basic fibroblast growth factor (bFGF) and markedly more robust responses to interleukin-6 and 8-Br-cAMP, has been subcloned from the rat PC12 cell line. The enhanced responsiveness to NGF in E2 cells is not due to receptor overexpression as judged by TrkA protein levels and tyrosine kinase activity, but may be associated with the increased and prolonged tyrosine phosphorylation of ERK1 (extracellular signal regulated kinase 1) and ERK2. The rapid morphological differentiation induced by different growth factors in E2 cells is mediated in a transcription-independent manner suggesting that E2 cells may constitutively express some differentiation-associated molecules that allow direct entry into the neuronal program.

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AnimalsCalcium-Calmodulin-Dependent Protein KinasesCell DifferentiationDactinomycinEpidermal Growth FactorFibroblast Growth Factor 2Gene ExpressionGrowth SubstancesMembrane GlycoproteinsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesNerve Growth FactorsNeuritesPC12 CellsPhosphotyrosineProto-Oncogene ProteinsRNA, MessengerRatsReceptor Protein-Tyrosine KinasesReceptor, Nerve Growth FactorReceptor, trkAReceptors, Nerve Growth FactorTranscription, GeneticTyrosineJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Y Y Wu, R A Bradshaw. 1995. PC12-E2 cells: a stable variant with altered responses to growth factor stimulation.. https://doi.org/10.1002/jcp.1041640310

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