Nonradioactive assay for sequence-specific DNA binding proteins.

M L Gubler; P Abarzúa

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PubMed · 7546700

Nonradioactive assay for sequence-specific DNA binding proteins.

M L Gubler·P Abarzúa

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Abstract

A nonradioactive functional assay was developed to quantitate DNA binding proteins. The assay was designed to allow the use of 96-well microplates for high sample throughput. We show that the assay can measure sequence-specific DNA binding of purified proteins as well as DNA binding activity present in whole cell extracts. By using a temperature-sensitive DNA binding protein, we demonstrate that DNA binding as measured by this novel assay correlates with the biological function of the protein.

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M L Gubler, P Abarzúa. 1995. Nonradioactive assay for sequence-specific DNA binding proteins.. https://pubmed.ncbi.nlm.nih.gov/7546700/

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