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alpha-Crystallin polypeptides in developing chicken lens cells.

A de Maria·C Arruti
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Abstract

We provide evidence that the different cells that form the chicken lens have isoelectric variants of alpha-crystallins at early and late developmental stages. We separated the alpha A and alpha B-crystallin subclasses by sodium dodecylsulphate polyacrylamide gel electrophoresis and then further resolved each by isoelectric focusing and assays with specific anti alpha-crystallin antibodies. We found that the annular pad, cortical and nuclear fibers, as well as the epithelial cells, contain alpha A and alpha B native chains and their respective isoelectric variants. These results on adult and embryonic lenses obtained a short time after the onset of alpha-crystallin expression suggest that lens cells, having different phenotypes, are able to produce post-translational modifications of the alpha A and alpha B chains as a part of their developmental program.

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AnimalsBlotting, WesternChick EmbryoChickensCrystallinsElectrophoresis, Polyacrylamide GelIsoelectric FocusingLens, CrystallinePeptidesProtein Processing, Post-TranslationalJournal ArticleResearch Support, Non-U.S. Gov't

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A de Maria, C Arruti. 1995. alpha-Crystallin polypeptides in developing chicken lens cells.. https://doi.org/10.1016/s0014-4835(05)80038-7

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