Protection against mycoplasma infection using expression-library immunization.

M A Barry; W C Lai; S A Johnston

doi:10.1038/377632a0

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Protection against mycoplasma infection using expression-library immunization.

M A Barry·W C Lai·S A Johnston

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Abstract

As is evident from the human immunodeficiency virus epidemic, there is no systematic method for producing a vaccine. Genetic immunization is a new approach to vaccine production that has many of the advantages of live/attenuated pathogens but no risk of infection. It involves introducing DNA encoding a pathogen protein into host cells and has shown promise in several disease models. Here we describe a new method for vaccine development, expression-library immunization, which makes use of the technique of genetic immunization and the fact that all the antigens of a pathogen are encoded in its DNA. An expression library of pathogen DNA is used to immunize a host thereby producing the effects of antigen presentation of a live vaccine without the risk. We show that even partial expression libraries made from the DNA of Mycoplasma pulmonis, a natural pathogen in rodents, provide protection against challenge from the pathogen. Expression library immunization may prove to be a general method for vaccination against any pathogen.

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Animals Antibodies, Bacterial Base Sequence DNA, Bacterial DNA, Recombinant Gene Library Humans Lung Lung Diseases Mice Molecular Sequence Data Mycoplasma Infections Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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M A Barry, W C Lai, S A Johnston. 1995-10-19. Protection against mycoplasma infection using expression-library immunization.. https://doi.org/10.1038/377632a0

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