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PubMed · 7570003

Serial analysis of gene expression.

V E Velculescu·L Zhang·B Vogelstein·K W Kinzler
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Abstract

The characteristics of an organism are determined by the genes expressed within it. A method was developed, called serial analysis of gene expression (SAGE), that allows the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate this strategy, short diagnostic sequence tags were isolated from pancreas, concatenated, and cloned. Manual sequencing of 1000 tags revealed a gene expression pattern characteristic of pancreatic function. New pancreatic transcripts corresponding to novel tags were identified. SAGE should provide a broadly applicable means for the quantitative cataloging and comparison of expressed genes in a variety of normal, developmental, and disease states.

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Base SequenceCloning, MolecularDNA Restriction EnzymesDNA, ComplementaryGene ExpressionGene LibraryGenetic TechniquesHumansMolecular Sequence DataPancreasPolymerase Chain ReactionRNA, MessengerJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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V E Velculescu, L Zhang, B Vogelstein, K W Kinzler. 1995-10-20. Serial analysis of gene expression.. https://doi.org/10.1126/science.270.5235.484

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