A simple, efficient technique for freezing Tetrahymena thermophila.

D Cassidy-Hanley; H R Smith; P J Bruns

doi:10.1111/j.1550-7408.1995.tb05897.x

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A simple, efficient technique for freezing Tetrahymena thermophila.

D Cassidy-Hanley·H R Smith·P J Bruns

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Abstract

We have developed a simple, efficient procedure for the long term freezing of Tetrahymena thermophila in liquid nitrogen. This technique yields excellent recovery of viable cells with all strains tested and does not require the use of a controlled rate low temperature freezer. To optimize the freezing technique, we have examined the effects of varying a number of parameters, including the physiological state of the cells prior to freezing, the time of exposure to cryoprotectant, and the rate of freezing and thawing. The frequency of viable cell recovery following freezing using this technique has been tested for a variety of different cell lines.

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Animals Cryopreservation Cryoprotective Agents Dimethyl Sulfoxide Tetrahymena thermophila Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

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D Cassidy-Hanley, H R Smith, P J Bruns. A simple, efficient technique for freezing Tetrahymena thermophila.. https://doi.org/10.1111/j.1550-7408.1995.tb05897.x

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