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PubMed · 7584845

Electrophoretic polymorphism in rabbit tear lysozyme.

A M Saleh·I M Ibrahimi
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Abstract

Rabbit tears were found to contain two lysozymes which differed in their electrophoretic mobility and were designated tear lysozymes 1 and 2. Rabbit tear lysozyme 1 was purified to homogeneity by conventional purification methods. It was found to be distinct from other known mammalian c-type lysozymes, rabbit tear lysozyme 2 and the major rabbit gastrointestinal lysozyme. The activity profile is centered around the neutral region with an optimum of 7 which is slightly lower than that for chicken lysozyme. The thermal stability as well as inhibition profiles by the substrate analogues, N-acetylglucosamine (NAG) and chitotetraose (NAG)4 are comparable to those of chicken lysozyme. Based on its molecular weight and catalytic properties this isozyme is classified as a c-type lysozyme.

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AnimalsAntibody FormationCatalysisElectrophoresis, Polyacrylamide GelEnzyme StabilityFemaleIsoenzymesMaleMuramidasePolymorphism, GeneticRabbitsTearsJournal ArticleResearch Support, Non-U.S. Gov't

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A M Saleh, I M Ibrahimi. 1995. Electrophoretic polymorphism in rabbit tear lysozyme.. https://doi.org/10.1016/0305-0491(95)00047-c

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