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PubMed · 7587747

Lymphocyte subset determination using a hematology analyzer.

J C Hudson·R F Brunhouse·C Garrison·C M Rodriguez·R Zwerner·T R Russell
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Abstract

Anti-CD4 antibody (T4)-coated microspheres were used to label CD4 cells in whole blood. The mixture was lysed and analyzed by a modified Coulter VCS hematology analyzer, which differentiated microsphere-labeled cells by a change in Coulter volume, conductance, and light scatter. %CD3+/CD4+ fluorescent values from a profile were compared to %CD4 values using the VCS-microsphere method. CD3 gating was used to exclude CD4+ monocytes from the 90LS-FALS lymphocyte gate. The results correlated well (R = 0.996). The percentage of CD4+ lymphocytes from profile scatterplots and VCS scatterplots showed a line of regression close to the equivalence line (n = 76, slope = 0.96) when CD3 gating was used for the profile. These results suggest that CD3 gating, though necessary for 90LS-FALS scatterplots, may not be necessary for volume-conductance-light scatterplots.

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Antibody SpecificityAutoanalysisBinding, CompetitiveBlood DonorsCell SizeElectric ConductivityFemaleFlow CytometryHIV SeropositivityHematologyHumansLightLymphocyte SubsetsMaleMicrospheresScattering, RadiationClinical TrialComparative StudyJournal ArticleRandomized Controlled Trial

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J C Hudson, R F Brunhouse, C Garrison, C M Rodriguez, R Zwerner, T R Russell. 1995-06-15. Lymphocyte subset determination using a hematology analyzer.. https://doi.org/10.1002/cyto.990220212

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