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Structure/function analysis of human interleukin 5 and its receptor.

J Tavernier·S Cornelis·R Devos·Y Guisez·G Plaetinck·J Van der Heyden
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Abstract

We have performed a detailed structure-function analysis of human interleukin 5 (hIL5) and its receptor. By testing a hIL5 mutant panel in a solid phase binding assay and a proliferation assay using hIL5 dependent cell-lines, areas on hIL5 involved in either the receptor alpha-subunit interaction or in receptor activation were identified. Epitope mapping data of a neutralizing and a non-neutralizing monoclonal antibody were in agreement with the mutant analysis. hIL5 binding areas on the IL5R alpha-subunit were identified by interspecies chimaera analysis. Finally, hIL5 mutants with reduced receptor activation potential have antagonistic properties.

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AnimalsHumansInterleukin-5MiceMolecular StructureMutationPeptide FragmentsReceptors, InterleukinReceptors, Interleukin-5Structure-Activity RelationshipJournal ArticleReview

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J Tavernier, S Cornelis, R Devos, Y Guisez, G Plaetinck, J Van der Heyden. 1995. Structure/function analysis of human interleukin 5 and its receptor.. https://doi.org/10.1007/978-3-0348-7276-8_3

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