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PubMed · 761373

Immobilized enzymes in continuous-flow analysis.

M Werner·R J Mohrbacher·C J Riendeau·E Murador·S Cambiaghi
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Abstract

Glucose oxidase, uricase, and urease were immobilized on the interior surface of activated polyamide tubing. The shelf-life of such enzyme bearing tubes was at least six months. The tubes were used for continuous-flow analysis of glucose, uric acid, and urea with conventional systems and with hybrid micro-scale systems in which modules of different manufacture were combined. The length of enzyme-bearing tube required for each system was ascertained empirically. Each tube could be used for several thousand assays, but glucose oxidase-bearing tubes were more stable than urease- or uricase-bearing tubes. Results for patients' samples correlated well with results obtained by accepted methods.

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AutoanalysisBlood GlucoseDrug StabilityEnzymes, ImmobilizedGlucose OxidaseHumansMethodsMicrochemistryUrate OxidaseUreaJournal Article

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M Werner, R J Mohrbacher, C J Riendeau, E Murador, S Cambiaghi. 1979. Immobilized enzymes in continuous-flow analysis.. https://pubmed.ncbi.nlm.nih.gov/761373/

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