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Functional and structural interactions between osteoblastic and preosteoclastic cells in vitro.

S Z Orlandini·L Formigli·S Benvenuti·L Lasagni·A Franchi·L Masi·P A Bernabei·V Santini·M L Brandi
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Abstract

Osteoblasts are involved in the bone resorption process by regulating osteoclast maturation and activity. In order to elucidate the mechanisms underlying osteoblast/preosteoclast cell interactions, we developed an in vitro model of co-cultured human clonal cell lines of osteoclast precursors (FLG 29.1) and osteoblastic cells (Saos-2), and evaluated the migratory, adhesive, cytochemical, morphological, and biochemical properties of the co-cultured cells. In Boyden chemotactic chambers, FLG 29.1 cells exhibited a marked migratory response toward the Saos-2 cells. Moreover, they preferentially adhered to the osteoblastic monolayer. Direct co-culture of the two cell types induced: (1) positive staining for tartrate-resistant acid phosphatase in FLG 29.1 cells; (2) a decrease of the alkaline phosphatase activity expressed by Saos-2 cells; (3) the appearance of typical ultrastructural features of mature osteoclasts in FLG 29.1 cells; (4) the release into the culture medium of granulocyte-macrophage colony stimulating factor. The addition of parathyroid hormone to the co-culture further potentiated the differentiation of the preosteoclasts, the cells tending to fuse into large multinucleated elements. These in vitro interactions between osteoblasts and osteoclast precursors offer a new model for studying the mechanisms that control osteoclastogenesis in bone tissue.

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AdultCartilageCell AdhesionCell CommunicationCell MovementCells, CulturedFemaleFilaggrin ProteinsHumansMicroscopy, ElectronOsteoblastsOsteoclastsJournal ArticleResearch Support, Non-U.S. Gov't

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S Z Orlandini, L Formigli, S Benvenuti, L Lasagni, A Franchi, L Masi, P A Bernabei, V Santini, M L Brandi. 1995. Functional and structural interactions between osteoblastic and preosteoclastic cells in vitro.. https://doi.org/10.1007/bf00307956

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