Decrease of D-loop frequency in heart and cerebral hemispheres mitochondrial DNA of aged rat.

V Petruzzella; F Fracasso; M N Gadaleta; P Cantatore

doi:10.1007/bf02962143

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Decrease of D-loop frequency in heart and cerebral hemispheres mitochondrial DNA of aged rat.

V Petruzzella·F Fracasso·M N Gadaleta·P Cantatore

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Abstract

A quantitative analysis of the frequency of the supercoiled mitochondrial DNA molecules containing the D-loop in rat heart and cerebral hemispheres, at different ages, is presented. Both tissues of aged animals exhibit a remarkable reduction in the content of super-coiled D-loop containing molecules compared to the adults. This alteration could be responsible for the age-dependent reduction of mitochondrial DNA transcription previously observed in rat brain and heart.

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Aging Animals Blotting, Southern Brain Chemistry DNA, Mitochondrial DNA, Superhelical Electrophoresis, Polyacrylamide Gel Male Mitochondria Mitochondria, Heart Nucleic Acid Conformation Rats Journal Article Research Support, Non-U.S. Gov't

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V Petruzzella, F Fracasso, M N Gadaleta, P Cantatore. Decrease of D-loop frequency in heart and cerebral hemispheres mitochondrial DNA of aged rat.. https://doi.org/10.1007/bf02962143

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