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PubMed · 7637039

Efficient magnesium-dependent human immunodeficiency virus type 1 integrase activity.

A Engelman·R Craigie
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Abstract

The integrase protein from human immunodeficiency virus type 1 (HIV-1) has generally been reported to require Mn2+ for efficient in vitro activity. We have reexamined the divalent metal ion requirements of HIV-1 integrase and find that the protein is capable of promoting efficient 3' processing and DNA strand transfer with either Mn2+ or Mg2+. The metal ion preference depended upon the reaction conditions. HIV-1 integrase displayed significantly less nonspecific nuclease activity in reaction mixtures containing Mg2+ than it did under the previously described reaction conditions with mixtures containing Mn2+.

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Base SequenceDNA NucleotidyltransferasesDNA, ViralHIV-1IntegrasesKineticsMagnesiumManganeseModels, GeneticMolecular Sequence DataOligodeoxyribonucleotidesSubstrate SpecificityVirus IntegrationComparative StudyJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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A Engelman, R Craigie. 1995. Efficient magnesium-dependent human immunodeficiency virus type 1 integrase activity.. https://doi.org/10.1128/jvi.69.9.5908-5911.1995

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