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A defined system for in vitro lambda DNA packaging.

Y Hwang·M Feiss
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Abstract

We constructed a defined in vitro system for packaging lambda DNA which is composed of purified proheads, the noncapsid proteins terminase and gpFI, and the Escherichia coli DNA binding/bending protein IHF. The defined packaging system: (i) is free from endogenous ATP, DNAs, and DNases and (ii) packages 30% of the input mature lambda DNA efficiently. In this defined packaging system, IHF and gpFI make modest contributions to packaging efficiency. The defined packaging reactions showed that DNA packaging gave a linear response to the concentration of mature lambda DNA and terminase. DNA packaging showed a sigmoidal relationship with respect to the concentration of ATP and proheads.

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Adenosine TriphosphateBacterial ProteinsBacteriophage lambdaDNA, ViralDNA-Binding ProteinsEndodeoxyribonucleasesIntegration Host FactorsViral ProteinsJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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Y Hwang, M Feiss. 1995-08-20. A defined system for in vitro lambda DNA packaging.. https://doi.org/10.1006/viro.1995.1419

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