pBINPLUS: an improved plant transformation vector based on pBIN19.

F A van Engelen; J W Molthoff; A J Conner; J P Nap; A Pereira; W J Stiekema

doi:10.1007/bf01969123

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pBINPLUS: an improved plant transformation vector based on pBIN19.

F A van Engelen·J W Molthoff·A J Conner·J P Nap·A Pereira·W J Stiekema

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Abstract

We describe the construction of a new plant transformation vector, pBINPLUS, based on the popular pBIN19 vector. Improvements over pBIN19 include location of the selectable marker gene at the left T-DNA border, a higher copy number in E. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes.

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Agrobacterium tumefaciens Amino Acid Oxidoreductases Base Sequence Cloning, Molecular DNA, Bacterial Genetic Vectors Kanamycin Kinase Molecular Sequence Data Phosphotransferases (Alcohol Group Acceptor)Plants Plants, Genetically Modified Plasmids Replication Origin Transformation, Genetic Journal Article Research Support, Non-U.S. Gov't

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F A van Engelen, J W Molthoff, A J Conner, J P Nap, A Pereira, W J Stiekema. 1995. pBINPLUS: an improved plant transformation vector based on pBIN19.. https://doi.org/10.1007/bf01969123

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