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PubMed · 7656335

Shared T cell epitopes in epithelial tumors.

G E Peoples·R C Smith·D C Linehan·I Yoshino·P S Goedegebuure·T J Eberlein
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Abstract

We have previously shown the importance of human leukocyte antigen (HLA)-A2 and the proto-oncogene HER2/neu in the T cell recognition of ovarian cancer. Since these proteins are ubiquitously expressed in epithelial-derived tumors, we have acid-eluted HLA-bound peptides from ovarian cancers, fractionated the peptides, and reconstituted T cell epitopes on the HLA-A2+ T2 cell line to determine if common tumor-associated antigens exist among HLA-A2+, HER2/neu+ epithelial cancers. We demonstrate that tumor-specific cytotoxic T lymphocytes (CTL) generated from tumor-infiltrating lymphocytes isolated from three ovarian, two breast, and two non-small-cell lung cancers recognize at least three of the same peptide fractions from multiple elutions. One of these peptide fractions coelutes with a HER2/neu-derived peptide which has been shown recently to be recognized by these same CTL. These findings demonstrate that a common peptide-based tumor vaccine is theoretically possible for many different epithelial-derived cancers.

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Antigens, NeoplasmBreast NeoplasmsCarcinoma, Non-Small-Cell LungEpitheliumFemaleHLA-A2 AntigenHumansImmunity, CellularIn Vitro TechniquesLung NeoplasmsOvarian NeoplasmsPeptidesProto-Oncogene MasErb-b2 Receptor Tyrosine KinasesT-Lymphocytes, CytotoxicTumor Cells, CulturedJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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G E Peoples, R C Smith, D C Linehan, I Yoshino, P S Goedegebuure, T J Eberlein. 1995. Shared T cell epitopes in epithelial tumors.. https://doi.org/10.1006/cimm.1995.1171

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