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Decrease of NCAM expression and astrocyte-neurone interaction in long-term cultured astrocytes.

T Yazaki·H Asou·K Arimoto·S Toya·K Uyemura
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Abstract

In order to assess the characteristics of the older astrocyte, we obtained long-term cultured rat astrocytes (20 months) and examined the features of protein expression in relation to neuronal interaction. In short-term cultured astrocytes, NCAM expressed strongly in contrast to weak expression of laminin by both immunocytochemical and ELISA assay. On the contrary, in long-term cultured astrocytes, a marked decrease of NCAM expression was observed along with increased laminin expression compared with short-term cultured astrocytes. The long-term cultured astrocytes remained positive to anti-GFAP antibody and showed a much lower ability to interact with neurones than the short-term cultured astrocytes. NCAM may be one of the responsible molecules related to the astrocyte-neurone interaction in the developing and ageing nervous system.

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AnimalsAstrocytesCell AdhesionCell Adhesion Molecules, NeuronalCell DivisionCells, CulturedCellular SenescenceEnzyme-Linked Immunosorbent AssayFemaleFluorescent Antibody TechniqueGene ExpressionGlial Fibrillary Acidic ProteinLamininNeuronsRatsRats, WistarTime FactorsJournal ArticleResearch Support, Non-U.S. Gov't

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T Yazaki, H Asou, K Arimoto, S Toya, K Uyemura. 1995-05-30. Decrease of NCAM expression and astrocyte-neurone interaction in long-term cultured astrocytes.. https://doi.org/10.1097/00001756-199505300-00003

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