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The three-dimensional point spread functions of a microscope objective in image and object space.

L Tao·C Nicholson
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Abstract

The three-dimensional point spread function (3-D PSF) of an optical system in image space is distinguished from the 3-D PSF in object space and the relation between the two 3-D PSFs is derived. By using this relation one 3-D PSF can be easily obtained from the other. The 3-D PSFs are given in a single integral expression, which can be computed numerically. The results of this study can be used in 3-D image processing for microscopy and have been applied to the analysis of the diffusion of fluorescent molecules in a 3-D porous medium.

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AlgorithmsFourier AnalysisImage Processing, Computer-AssistedLensesMathematicsMicroscopyJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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BibTeXRIS

L Tao, C Nicholson. 1995. The three-dimensional point spread functions of a microscope objective in image and object space.. https://doi.org/10.1111/j.1365-2818.1995.tb03604.x

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