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Ultrasensitive bioanalytical assays using time-resolved fluorescence detection.

E F Dickson·A Pollak·E P Diamandis
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Abstract

This article reviews the use of time-resolved fluorimetric detection of lanthanide chelate luminescence as a detection method for ultrasensitive bioanalytical assays. Assay formats and detection methods, and the principle of time-resolved fluorimetric detection, are described. Detection systems, assay formats, reagents, and instrumentation for time-resolved fluorimetric detection are outlined. A review of published and commercially available immunoassays and DNA hybridization assays using time-resolved fluorimetric detection of lanthanide chelate luminescence is given.

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Chelating AgentsDNA ProbesFluoroimmunoassayImmunoassayMetals, Rare EarthNucleic Acid HybridizationTime FactorsJournal ArticleReview

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BibTeXRIS

E F Dickson, A Pollak, E P Diamandis. 1995. Ultrasensitive bioanalytical assays using time-resolved fluorescence detection.. https://doi.org/10.1016/0163-7258(94)00078-h

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