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PubMed · 7667636

CDC25 phosphatases as potential human oncogenes.

K Galaktionov·A K Lee·J Eckstein·G Draetta·J Meckler·M Loda·D Beach
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Abstract

Cyclin-dependent kinases (CDKs) are activated by CDC25 phosphatases, which remove inhibitory phosphate from tyrosine and threonine residues. In human cells, CDC25 proteins are encoded by a multigene family, consisting of CDC25A, CDC25B, and CDC25C. In rodent cells, human CDC25A or CDC25B but not CDC25C phosphatases cooperate with either Ha-RASG12V or loss of RB1 in oncogenic focus formation. Such transformants were highly aneuploid, grew in soft agar, and formed high-grade tumors in nude mice. Overexpression of CDC25B was detected in 32 percent of human primary breast cancers tested. The CDC25 phosphatases may contribute to the development of human cancer.

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AnimalsBreast NeoplasmsCell Cycle ProteinsCell DivisionCell Transformation, NeoplasticCells, CulturedGene ExpressionGenes, RetinoblastomaGenes, p53Genes, rasHumansIn Situ HybridizationMiceMice, Inbred BALB CMice, NudeMultigene FamilyNeoplasm TransplantationOncogenesPhosphoprotein PhosphatasesPrognosisTransfectionTumor Cells, Culturedcdc25 PhosphatasesJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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K Galaktionov, A K Lee, J Eckstein, G Draetta, J Meckler, M Loda, D Beach. 1995-09-15. CDC25 phosphatases as potential human oncogenes.. https://doi.org/10.1126/science.7667636

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