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PubMed · 7669915

Specific interaction between cyclophilin and cyclic peptides.

P Gallo·M Saviano·F Rossi·V Pavone·C Pedone·R Ragone·P Stiuso·G Colonna
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Abstract

Cyclophilin A, a peptidyl-prolyl cis-trans is isomerase that catalyzes the otherwise slow isomerization of Xaa-Pro imidic bond, specifically binds the immunosuppressant cyclosporin A. Herein we report evidence on binding of cyclolinopeptide A and its synthetic analogue, [Aib5,6-D-Ala8]cyclolinopeptide, to bovine cyclophilin A. Binding experiments were monitored by fluorescence, CD, and second-derivative spectroscopies, evidencing no remarkable rearrangement of protein structure organization. The possibility that cyclolinopeptide A could act as a substitute of cyclosporin A in the immunosuppression modulation is also briefly discussed.

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Amino Acid IsomerasesAnimalsCarrier ProteinsCattleChromatography, AffinityChromatography, GelCircular DichroismCyclosporineImmunosuppression TherapyKineticsPeptide FragmentsPeptides, CyclicPeptidylprolyl IsomeraseProtein BindingProtein ConformationSpectrometry, FluorescenceSpectrophotometryThymus GlandJournal Article

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P Gallo, M Saviano, F Rossi, V Pavone, C Pedone, R Ragone, P Stiuso, G Colonna. 1995. Specific interaction between cyclophilin and cyclic peptides.. https://doi.org/10.1002/bip.360360303

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