DNA-damage processing in a radiation-sensitive mouse cell line.

R L Warters; B W Lyons; D J Chen; K Sato

doi:10.1016/0921-8777(93)90061-k

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DNA-damage processing in a radiation-sensitive mouse cell line.

R L Warters·B W Lyons·D J Chen·K Sato

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Abstract

The induction and repair of radiation-induced DNA damage was assessed in 3 mouse cell lines, including the parental L cell line, a radiation-sensitive, SL3-147 mutant cell line and the H5 revertant to radiation resistance. The yield of neither radiation-induced DNA single- nor double-strand breaks could explain the variable sensitivity of the 3 cell lines. Closure of DNA single-strand breaks proceeded at a similar rate in both the L and SL3-147 cell lines. Closure of DNA double-strand breaks however was significantly slower and less complete in the SL3-147 cell line than in either of the radiation-resistant cell lines. The results are consistent with the increased radiation sensitivity of the SL3-147 cell line resulting from a defect in their ability to repair radiation-induced DNA double-strand breaks.

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Animals Cell Survival DNA DNA Damage DNA Repair Dose-Response Relationship, Radiation In Vitro Techniques L Cells Methylnitronitrosoguanidine Mice X-Rays Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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R L Warters, B W Lyons, D J Chen, K Sato. 1993. DNA-damage processing in a radiation-sensitive mouse cell line.. https://doi.org/10.1016/0921-8777(93)90061-k

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