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PubMed · 7708695

Two helices plus a linker: a small model substrate for eukaryotic RNase P.

G Carrara·P Calandra·P Fruscoloni·G P Tocchini-Valentini
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Abstract

Using precursor tRNA molecules to study RNA-protein interactions, we have identified an RNA motif recognized by eukaryotic RNase P (EC 3.1.26.5). Analysis of circularly permuted precursors indicates that interruptions in the sugar-phosphate backbone are not tolerated in the acceptor stem, in the T stem-loop, or between residues A-9 and G-10. Prokaryotic RNase P will function with a minihelix consisting of the acceptor stem connected directly to the T stem-loop. Eukaryotic RNase P cannot use such a minimal substrate unless a linker sequence is added in the gap where the D stem and anticodon stem-loop were deleted.

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AnimalsBase SequenceDNA-Directed RNA PolymerasesEndoribonucleasesKineticsModels, StructuralMolecular Sequence DataMutagenesisNucleic Acid ConformationRNA PrecursorsRNA, CatalyticRNA, Transfer, PheRibonuclease PSubstrate SpecificityTemplates, GeneticTranscription, GeneticViral ProteinsXenopus laevisComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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G Carrara, P Calandra, P Fruscoloni, G P Tocchini-Valentini. 1995-03-28. Two helices plus a linker: a small model substrate for eukaryotic RNase P.. https://doi.org/10.1073/pnas.92.7.2627

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