PubMed HealthSearch

PubMed · 7720225

Fluorescence-activated cytometry cell sorting based on immunological recognition.

Abstract

The source did not provide an abstract. Follow the original record for more information.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

M Assenmacher, R Manz, S Miltenyi, A Scheffold, A Radbruch. 1995. Fluorescence-activated cytometry cell sorting based on immunological recognition.. https://doi.org/10.1016/0009-9120(94)00063-2

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

Use of magnetic techniques for the isolation of cells.

Magnetic separation is an emerging technology using magnetism, sometimes in combination with conventional separation or identification methods, to purify cells, cell organelles and biologically active compounds (nucleic acids, proteins, xenobiotics) directly from crude samples. Several magnetic separation procedures have been developed to isolate target cells specifically. The purpose of this short review is to summarize various methodologies, strategies and materials which can be employed for the selection and separation of target cells with the help of magnetic field and thus to help the novices in this field to be able to orient themselves in vast amount of literature available. Immunomagnetic separations employing specific antibodies to label the target cells represent the most often used approach and are discussed in detail.

Cell Separation

Human conjunctival mast cells: expression of Fc epsilonRI, c-kit, ICAM-1, and IgE.

OBJECTIVE: To characterize the expression and regulation of conjunctival mast cell surface receptors important in allergic inflammation. METHODS: Mast cells were isolated from human conjunctival tissues of cadavers. Mast cell surface markers were identified using flow cytometry with antibodies to IgE, Fc epsilonRI, c-kit, and intercellular adhesion molecule-1 (ICAM-1). We evaluated the effect of 24-hour tumor necrosis factor alpha (TNF-alpha) or interleukin 4 (IL-4) incubation on the expression of mast cell c-kit, ICAM-1, and surface-bound IgE. RESULTS: Staining of mast cells (c-kit and/or tryptase positive) yielded positive results for all of the variables measured. The intensity of mast cell c-kit staining increased with TNF-alpha incubation, but decreased below that of unstimulated mast cells when incubated with IL-4. Anti-ICAM-1 and anti-IgE staining were increased over that of unstimulated cells when incubated with TNF-alpha or IL-4. CONCLUSIONS: In this model, TNF-alpha up-regulates mast cell surface receptors and cell-bound IgE. Interleukin 4 up-regulates mast cell ICAM-1 and cell-bound IgE, but down-regulates c-kit. CLINICAL RELEVANCE: Conjunctival mast cells play a critical role in the pathogenesis of atopic ocular disease. Characterization of the expression and regulation of mast cell surface receptors is important to the development of potential novel treatments for ocular inflammation.

Cell Separation

An automatic temperature-control system for solutions in free flow.

We describe a temperature-control system for solutions in free flow, suitable for electrophysiological or optical studies of isolated cells, natural epithelia or cell culture monolayers. The system is small enough to be located close to the preparation and was designed specifically to be coupled to the inlets of a modified, continuous-flow Ussing chamber, allowing rapid change of the solutions bathing tissue surfaces. The system consists of a highly compact monoblock heating unit and a control circuit. Solutions from different reservoirs, kept at room temperature or lower (from an ice bath), can be rapidly switched at the inlet of the heating unit by manually or electrically actuated microvalves without affecting the temperature of the fluid leaving the heating unit. The control unit consists of a bead thermistor firmly placed close to the heating unit outlet and an electronic circuit which is basically a proportional controller. This unit continuously regulates the electric current through the Ni-Cr heater, keeping the temperature of the fluid leaving the heating unit constant at a preset value. The system allows control of fluid temperature (normally 37 degrees C) for flow rates in the range of 1.0 ml/min to 12 ml/min. However, the temperature can be set at any value above that of the incoming fluid.

Cell Separation