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PubMed · 7720245

HLA class II genotyping: two assay systems compared.

J Thonnard·F Deldime·M Heusterspreute·B Delepaut·F Hanon·M De Bruyère·M Philippe
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Abstract

In the last few years, a variety of DNA-based human leukocyte antigen (HLA) typing methods have emerged, revealing the extreme polymorphism of HLA genes. This polymorphism makes it difficult for a clinical laboratory to establish the best HLA typing strategy. In this study we have compared two techniques for performing HLA-DRB typing: a commercial rapid assay based on the polymerase chain reaction (PCR) followed by reverse dot-blot hybridization of the PCR products (the Inno-LiPA assay), and a method based on PCR followed by restriction fragment length polymorphism analysis. We found that both methods provide reliable results with a high rate of concordance (97%) and that Inno-LiPA is convenient for large-scale routine typing. However, if a high-resolution allelic typing is required, each method lacks accuracy but using them in association improves the accuracy of the results.

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AllelesGenotypeHistocompatibility Antigens Class IIHumansNucleic Acid HybridizationPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthSensitivity and SpecificityTemperatureComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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J Thonnard, F Deldime, M Heusterspreute, B Delepaut, F Hanon, M De Bruyère, M Philippe. 1995. HLA class II genotyping: two assay systems compared.. https://pubmed.ncbi.nlm.nih.gov/7720245/

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