Serrate expression can functionally replace Delta activity during neuroblast segregation in the Drosophila embryo.

Y Gu; N A Hukriede; R J Fleming

doi:10.1242/dev.121.3.855

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Serrate expression can functionally replace Delta activity during neuroblast segregation in the Drosophila embryo.

Y Gu·N A Hukriede·R J Fleming

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Abstract

Serrate and Delta encode structurally related proteins in D. melanogaster that bind within a common extracellular region on the NOTCH receptor molecule. We used ectopic expression to determine if SERRATE could mediate in vivo functions parallel or antagonistic to those proposed for the putative NOTCH ligand DELTA. Our results demonstrate that Serrate can replace Delta gene function during embryonic neuroblast segregation and that expression of Serrate leads to a NOTCH-dependent suppression of achaete expression in proneural clusters. Our findings strongly suggest that SERRATE functions as an alternative ligand capable of NOTCH activation.

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Animals Calcium-Binding Proteins Drosophila Proteins Drosophila melanogaster Embryonic Induction Gene Expression Immunohistochemistry In Situ Hybridization Insect Hormones Intercellular Signaling Peptides and Proteins Intracellular Signaling Peptides and Proteins Jagged-1 Protein Membrane Proteins Mutagenesis Nervous System Receptors, Notch Serrate-Jagged Proteins Journal Article Research Support, Non-U.S. Gov't

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Y Gu, N A Hukriede, R J Fleming. 1995. Serrate expression can functionally replace Delta activity during neuroblast segregation in the Drosophila embryo.. https://doi.org/10.1242/dev.121.3.855

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